36th International Symposium on Preparative and Process Chromatography
Philadelphia, PA, United States May 28-31, 2024
Program
Tuesday May 28, 2024
09:00 am
Morning Workshops
Start of parallel sessions
Workshop 1
Workshop 2
Workshop 3
Workshop 1
Small Molecule Batch and Continuous Chromatography
Dr Geoffrey COX B. INDEPENDENT CONSULTANT HPLC, SMB & SFC, Wallingford, United States Dr Olivier DAPREMONT SK PHARMTECO, Rancho Cordova, United States
Dr Olivier DAPREMONT
Dr Dapremont received his Ph.D. degree in Chemical Engineering and Applied Chemistry in Simulated Moving Bed technology (SMB) and chiral applications in 1997 from the University of Pierre and Marie Curie in Paris France.
He started his career, in 1992, developing SMB technology for Prochrom R&D;, France. In 1997, He joined Chiral Technologies Europe, France, were he was in charge of the kilo lab for SMB chiral separations service.
He joined Aerojet Fine Chemicals, now AMPAC Fine Chemicals (AFC) in Rancho Cordova, CA, at the beginning of 2001. At AFC, Dr. Dapremont is in charge of the development of continuous processes for APIs and intermediates including chromatographic processes as well as implementing flow chemistry and related techniques at large scale. To this day, he has developed and implemented over 50 chiral and non-chiral separations using SMB from kilogram to multi ton scale.
Dr. Dapremont is author and co-author of several articles on preparative chromatography and SMB applications in various scientific journals and magazines. He is co-inventor on multiple patents using SMB as a purification step for APIs and he is a recognized expert in the field for the past 25 years.
Dr. Dapremont is also a member of the Organizing Committee of the Prep Symposium conference and a member of the Scientific Committee of the SPICA conference.
Workshop 2
Bioseparation - Proteins
Dr Khaled MRIZIQ PROCESS CHROMATOGRAPHY CONSULTANT , Knoxville, United States Ms Sharon BOLA BIO-RAD LABORATORIES, Hercules, United States
Ms Sharon BOLA
Sharon Bola is a Global Product Manager with the Process Chromatography resins group at Bio-Rad Laboratories. She holds a MS degree in Pharmacology from St John�s University, NY. Prior to transitioning into her current role, she started her career working in downstream processing groups at major Biopharmaceutical companies on the east and west coast. She has had hands-on experience in the purification of proteins including monoclonal antibodies from research to manufacturing scales. She is a content marketer at Bio-Rad and enjoys learning, teaching and understanding customer�s needs.
Workshop 3
Oligo/Peptides Purification Basics
Dr Chiara DE LUCA UNIVERSIT� DEGLI STUDI DI FERRARA, Ferrara, Italy Dr Mattia SPONCHIONI POLITECNICO DI MILANO, Milano, Italy
01:00 pm
End of Morning Workshops
02:00 pm
Afternoon Workshops
Start of parallel sessions
Workshop 4
Workshop 5
Workshop 6
Workshop 4
mRNA and Viral Vector Purification
Dr Urh CERNIGOJ BIA SEPARATIONS, Slovenia Dr Stefano MENEGATTI NC STATE UNIVERSITY & LIGATRAP TECHNOLOGIES, Raleigh, United States
Alisa Strobel has been working as a scientist at MilliporeSigma in Darmstadt, Germany since 2020 with a focus on chromatography product development. She has experience with the characterization and application testing of separation materials with different modalities and is specialized in the purification of pDNA and viral vectors. She holds a Master of Science degree in Biotechnology - Bioprocess Development from the University of Applied Sciences Mannheim, Germany.
Workshop 5
SFC
Dr Gerard ROSSE PIC SOLUTION, San Diego, United States
Workshop 6
HTS and Modeling
Dr Nick VECCHIARELLO UNIVERSITY OF VIRGINIA, Charlottesville, United States Dr John WELSH RIVANNA BIO PROCESS, Charlottesville, United States
03:30 pm
Registration
FOYER LEVEL 2
06:00 pm
End of Afternoon Workshops
06:00 pm
Welcome Reception in the Exhibition Hall
Wednesday May 29, 2024
07:00 am
Registration
FOYER LEVEL 2
07:00 am
Start of parallel sessions
Vendor Seminar by emp BIOTECH
Vendor Seminar by JNC Corporation
Vendor Seminar by Tosoh Bioscience
Vendor Seminar by emp BIOTECH
07:00 am
SMART Chromatography� - Feed Stream Clarification and Primary Purification in a Single Unit Operation
Vendor Seminar by JNC Corporation
07:00 am
How to Overcome Challenges in the Purification of Biomacromolecules with Cellufine and MLP Technologies
Vendor Seminar by Tosoh Bioscience
07:00 am
Multi-Column Chromatography: A Solution for Modern Biomanufacturing Challenges
Breakfast Vendor Seminars
08:15 am
Opening Remarks
Dr Olivier DAPREMONT SK PHARMTECO, Rancho Cordova, United States
ROOM COMMONWEALTH BCD
Session 1: Bioprocess
ROOM COMMONWEALTH BCD
Session Chair
Dr Stefano MENEGATTI NC STATE UNIVERSITY & LIGATRAP TECHNOLOGIES, Raleigh, United States
Molecular Bioprocessing: Biophysics, Simulations and Expedited Process Development (PL01)
Prof. Steven CRAMER M RENSSELAER POLYTECHNIC INSTITUTE, Troy, United States
Prof. Steven CRAMER M
Professor Steven Cramer is an Institute Professor at Rensselaer Polytechnic Institute, Troy, New York. The Cramer lab�s research over the past 37 years has helped to improve the state of the art of downstream bioprocessing and its successful implementation for the biomanufacturing of biological products. Current research in the Cramer lab using a combination of biophysics, molecular dynamics simulations and chromatography with ligand protein libraries has provided insights into the design of novel multimodal chromatographic systems. Recent work on integrated biomanufacturing and expedited process development is having an impact on how bioprocess development is carried out in the industry. The Cramer lab is now actively involved in gene therapy downstream biomanufacturing projects, continuous mRNA processing, and several big data modeling efforts. Professor Cramer has won numerous awards including the ACS National Award in Separations Science and Technology, the ACS BIOT Division�s Michaels Award in the Recovery of Biological Products, the Gaden award from the ACS BIOT Division, and several awards from RPI including the Wiley Distinguished Faculty Award and the School of Engineering Outstanding Professor and Research Excellence Awards. Cramer has been elected a member of the National Academy of Engineering and U.T. Austin�s Academy of Distinguished Chemical Engineers. He is also an elected fellow of the American Association for the Advancement of Science, American Institute of Chemical Engineers, the American Chemical Society, and the American Institute for Medical and Biological Engineering. He has published extensively in the field with 230 publications, and the 57 Ph.D. graduates from the Cramer lab have had a significant impact with many of his former students now playing key leadership roles in the state of the art of industrial bioprocessing at most of the major biopharmaceutical and bioseparations companies worldwide as well as academia.
09:00 am
Development of Rabbit scFv-based Immunoaffinity Chromatography for Purification of Human Kynureninase (OC01)
Prof. Yoichi KUMADA KYOTO INSTITUTE OF TECHNOLOGY, Kyoto, Japan
09:20 am
Purification of Therapeutic Proteins Expressed in Mammalian Hosts Using a Self-removing Affinity Tag (OC02)
Prof. David WOOD THE OHIO STATE UNIVERSITY, Columbus, United States
09:40 am
Improved Manufacturing and Characterization of a Fusogenic Oncolytic Virus (OC03)
Ms Rita FERNANDES IBET, Oeiras, Portugal
10:00 am
Coffee Break and Exhibition Sponsored by Asahi Kasei Bioprocess America Inc
MILLENIUM HALL
Session 2: Fundamentals
ROOM COMMONWEALTH BCD
Session Chair
Prof. Dorota ANTOS RZESZOW UNIVERSITY OF TECHNOLOGY, Rzeszow, Poland
10:20 am
A Mechanistic Modeling Strategy to Strengthen Serotype Specific AAV Full/Empty Process Optimization and Robustness Screening (OC04)
Dr Tatjana TRUNZER CYTIVA, Karlsruhe, Germany
Dr Tatjana TRUNZER
Tatjana is a scientist in the field of mechanistic modeling, researching and developing the GoSilico� Chromatography Modeling Software at Cytiva. She has extensive background with in vitro and in silico chromatography process development, flow-through operations and scaling. She has studied for a Bachelor and Master of Sciences in Chemical Engineering, and a Dr.-Ing. in biopharmaceutical process development. Tatjana enjoys bringing novel in silico tools to the industry, and searching for an improved understanding of the mechanistic behavior of bioprocessing, enabling rapid process development.
10:40 am
The New Frontier? Exploring Innovative Magnetic Separation with Mechanistic Models (OC05)
Mr Marko TESANOVIC TU MUNICH, M�nchen, Germany
Mr Marko TESANOVIC
Marko Tesanovic's academic journey began with a bachelor's degree in Mechanical Engineering from RWTH Aachen University. His academic pursuits further led him to acquire two master's degrees: an M.Sc. in Process Engineering from the RWTH Aachen, and an M.Sc. in Management Science & Engineering from Tsinghua University, Beijing.
Currently, as a doctoral candidate at the Technical University of Munich, he is engaged with the Chair of Bioseparation Engineering. His research centers on advancing high-gradient magnetic separation technologies within the field of biotechnology, with a particular focus on developing mathematical models and a digital infrastructure for magnetic separators. This work is aimed at enabling in silico methods that could facilitate future digital twin technologies. In addition to his research, he is involved in a start-up specializing in software for downstream processing, which has received funding from the German government in 2023
11:00 am
Adapting High-throughput Technologies for Enhanced Mechanistic Modeling in Chromatography: A Novel Calibration Approach Combining KP And Robocolumn (OC06)
Dr Chyi-Shin CHEN CHUGAI PHARMACEUTICAL, Tokyo, Japan
Dr Chyi-Shin CHEN
Chyi-Shin Chen is a scientist with experience in bioprocesses and data science. She is currently employed at Chugai Pharmaceutical, focusing on downstream process technology development. Prior to this, she served as a Senior Process Engineer at Berkeley National Laboratory, specializing in bioprocess engineering. Chen earned her PhD in chromatography modeling from Yamaguchi University. In her present role at Chugai Pharmaceutical, she is working on the development of downstream process technologies, including in silico models and digital infrastructure design, to expedite process development.
11:20 am
Elucidating the Exclusion Mechanism in Cation-exchange Chromatography with High-throughput Techniques and Mechanistic Modeling to Improve Performance of Therapeutic Antibody Polishing (OC07)
Dr Scott ALTERN ABBVIE, Worcester, United States
Dr Scott ALTERN
Hi! My name is Scott H. Altern. I recently graduated from Rensselaer Polytechnic Institute with my Ph.D. from Prof. Steven Cramer�s group. The focus of my thesis was on mechanistic modeling of multimodal chromatography. Since graduation, I joined the Purification Development team at AbbVie as a Senior Scientist. My work at AbbVie has been centered on high-throughput experimentation and mechanistic modeling to accelerate process development workflows. I am very thankful for the opportunity to be here at PREP!
11:40 am
Mechanistic Modeling of mAbs And Host-cell Protein Impurities in Protein a Chromatography (OC08)
Mr Soumitra BHOYAR UNIVERSITY OF DELAWARE, Newark, United States
Mr Soumitra BHOYAR
Soumitra is a 5th year graduate student at the University of Delaware Chemical and Biomolecular Engineering department, working under Dr. Abraham Lenhoff. His skills include chromatography, column modelling and biophysical characterization of proteins.
12:00 pm
Poster Flash Presentation
ROOM COMMONWEALTH BCD
12:20 pm
Lunch and Exhibition
MILLENIUM HALL & VENDOR SEMINAR ROOMS
Lunch Vendor Seminars
12:20 pm
Start of parallel sessions
Vendor Seminar by Bio-Rad Laboratories
Vendor Seminar by Pic Solution
Vendor Seminar by SK Pharmteco
Vendor Seminar by Sunresin
Vendor Seminar by YMC America Inc
Vendor Seminar by Bio-Rad Laboratories
12:20 pm
Unlocking the Potential: Advancements and Strategies in Downstream Biomolecule Purification
Vendor Seminar by Pic Solution
12:20 pm
Twenty Years of Supercritical Fluid Chromatography Expertise
Vendor Seminar by SK Pharmteco
12:20 pm
The Importance of Chromatography in the Advanced Therapy Landscape � A Case Study of Plasmid DNA and Viral Vector Manufacturing
Vendor Seminar by Sunresin
12:20 pm
To Affinity and Beyond: Innovations in high-quality Protein A, Ion Exchange, HIC, Mixed Mode, and Size Exclusion chromatography resins for more cost-effective biomolecule purification
Vendor Seminar by YMC America Inc
12:20 pm
An Overview of Twin-Column Prep Chromatography Techniques
01:35 pm
Poster Session 1
MILLENIUM HALL
03:00 pm
Start of parallel sessions
Session 3A: Continuous Processing
Session 3B: Stationary Phases 1
Session 3A: Continuous Processing
Session Chairs
Dr Xuan HAN AMGEN, Cambridge, United States Prof. Abraham M. LENHOFF UNIVERSITY OF DELAWARE, Newark, United States
03:00 pm
Continuous Separation of Enantiomers in SMB Chromatography with Achiral Stationary Phase (OC09)
Dr Wojciech MAREK RZESZOW UNIVERSITY OF TECHNOLOGY, Rzeszow, Poland
Dr Wojciech MAREK
Since 2014, I work as an assistant professor and academic teacher at Rzesz�w [��shoov] University
of Technology, Faculty of Chemistry, Department of Chemical and Process Engineering in the
research group of prof. Dorota Antos. My scientific work mainly concerns downstream processing
of proteins and bioactive compounds with its mathematical modeling and optimization. I work
on improvement of various separation techniques like liquid chromatography (IEC, HIC, SEC, AC,
RP), aqueous two-phase extraction (ATPE), centrifugal partition chromatography (CPC) and SMB. I
also carried out scholarships and international projects in cooperation with the research group of
prof. Andreas Seidel-Morgenstern at Max Planck Institute for Dynamics of Complex Technical
Systems in Magdeburg, Germany and with the research group of prof. Alois Jungbauer at
University of Natural Resources and Life Sciences in Vienna, Austria
03:20 pm
Adapting the Separation Parameters to Address an Unexpected Impurity in a Validated Process (OC10)
Dr Olivier DAPREMONT SK PHARMTECO, Rancho Cordova, United States
Dr Olivier DAPREMONT
Dr Dapremont received his Ph.D. degree in Chemical Engineering and Applied Chemistry in Simulated Moving Bed technology (SMB) and chiral applications in 1997 from the University of Pierre and Marie Curie in Paris France.
He started his career, in 1992, developing SMB technology for Prochrom R&D;, France. In 1997, He joined Chiral Technologies Europe, France, were he was in charge of the kilo lab for SMB chiral separations service.
He joined Aerojet Fine Chemicals, now AMPAC Fine Chemicals (AFC) in Rancho Cordova, CA, at the beginning of 2001. At AFC, Dr. Dapremont is in charge of the development of continuous processes for APIs and intermediates including chromatographic processes as well as implementing flow chemistry and related techniques at large scale. To this day, he has developed and implemented over 50 chiral and non-chiral separations using SMB from kilogram to multi ton scale.
Dr. Dapremont is author and co-author of several articles on preparative chromatography and SMB applications in various scientific journals and magazines. He is co-inventor on multiple patents using SMB as a purification step for APIs and he is a recognized expert in the field for the past 25 years.
Dr. Dapremont is also a member of the Organizing Committee of the Prep Symposium conference and a member of the Scientific Committee of the SPICA conference.
03:40 pm
Continuous Ternary Separation Using Double-layer Simulated Moving Bed Chromatography (OC11)
Dr Ju Weon LEE MAX-PLANCK-INSTITUT FUR DYNAMIK KOMPLEXER TECHNISCHER SYSTEME, Magdeburg, Germany
04:00 pm
Twin-column Chromatography for Isolating mAb Isoforms and Other Pharmaceutical Minor Components (OC12)
J Preston, PhD is presently the Director of Separation Sciences at YMC America. He has over 25 years of industrial experience that has been focused on chromatography in the pharmaceutical industry. Dr Preston spent over 8 years at Amgen, 3 years at Pfizer / Pharmacia / GD Searle and 3 years with Abbott Labs. Prior to joining YMC America, Dr. Preston spent 9 years at the Phenomenex Corporation as the Manager and Principal Scientist in their applications lab, and as the Global Product manager for their Chiral, Prep and Bulk product lines. Dr. Preston has a Doctorate in Analytical Chemistry from the University of Illinois at Urbana-Champaign, an MS in Analytical Chemistry from East Texas State, and a BS in Chemistry from Central Michigan University. J has published numerous peer reviewed articles and has given many presentations at national and international meetings.
Session 3B: Stationary Phases 1
Session Chairs
Mrs Sunitha KANDULA MERCK GROUP, Union City , United States
03:00 pm
Innovative Mixed Mode Resin for Efficient Purification of Complex Antibody Formats: Impurity Removal and High Purity Bispecific Antibody Recovery (OC13)
Alisa Strobel has been working as a scientist at MilliporeSigma in Darmstadt, Germany since 2020 with a focus on chromatography product development. She has experience with the characterization and application testing of separation materials with different modalities and is specialized in the purification of pDNA and viral vectors. She holds a Master of Science degree in Biotechnology - Bioprocess Development from the University of Applied Sciences Mannheim, Germany.
03:20 pm
New Monodispersed Silica and Hybrid Silica Bulk Media for Purification of Peptides and Oligonucleotides (OC14)
Dr WU CHEN NANOMICRO TECHNOLOGY, NEWARK, United States
Dr WU CHEN
Dr. Wu Chen joined NanoMicro Technology as CTO in January, 2022, in charge of developments of bulk media for purification market. Before that, he was a master scientist in Agilent Technologies in Wilmington, Delaware for 24 years, developing silica based packing materials for HPLC columns. He got his Ph.D. degree in organic chemistry in Iowa State University.
03:40 pm
Impact of Jetting and Batch Emulsification Resin Manufacturing Technologies on Pressure-flow Characteristics (OC15)
Mr Christopher GERBERICH GSK, King of Prussia, United States
Mr Christopher GERBERICH
Chris Gerberich joined GSK in 2014 as part of the biopharm downstream process development group and since then, he has contributed to the chromatography development for several early- and late-phase assets. He is a subject matter expert on mechanistic modeling of chromatography and advanced data analytics within GSK, and has employed these models and analytics to improve process understanding, optimize existing processes, and extract more information from experiments. Chris received his Master�s degree in biochemical engineering from Villanova University in 2019.
04:00 pm
Effect of Microstructure and Porosity on Compression of Methacrylate Polymers During Flow-through Applications (OC16)
Dr Ales PODGORNIK FACULTY OF CHEMISTRY AND CHEMICAL TECHNOLOGY, UNIVERSITY OF LJUBLJANA, Ljubljana, Slovenia
04:20 pm
Georges Guiochon - in Memoriam
ROOM COMMONWEALTH BCD
04:30 pm
Coffee Break and Exhibition Sponsored by Novasep Group, an Axplora Company
MILLENIUM HALL
05:00 pm
Start of parallel sessions
Session 4A: Process Analytical Technologies PAT
Session 4B: Stationary phases 2
Session 4A: Process Analytical Technologies PAT
Session Chairs
Prof. Massimo MORBIDELLI ARISTOTLE UNIVERSITY OF THESSALONIKI, Thessaloniki, Greece Dr Olivier DAPREMONT SK PHARMTECO, Rancho Cordova, United States
05:00 pm
Process Analytical Technology (PAT) Informing the Manufacture and Formulation of Monoclonal Antibody-based Products (OC17)
Ms Maria BRUQUE UNIVERSITY OF BIRMINGHAM, Birmingham, United Kingdom
05:20 pm
Size-exclusion Chromatography Sensor as a Process Analytical Technology for the Manufacturing Process of Monoclonal Antibodies (OC18)
Dr Fabrice GRITTI WATERS CORPORATION, MILFORD, United States
Dr Fabrice GRITTI
Fabrice G. Gritti received a Ph.D. in Chemistry and Physics of Condensed Matter from the University of Bordeaux I (France) in 2001. He then worked as a research scientist at the University of Tennessee (Knoxville, TN) from 2002 to until 2014 in the research group of Prof. Georges Guiochon. He joined Waters Corporation in 2015 where he is currently a consultant scientist.
Dr. Gritti�s main research interests involve liquid/solid adsorption thermodynamics and mass transfer in heterogeneous media used in the field of separation science. During the last 25 years, he has provided fundamental insights in preparative liquid chromatography and on the retention mechanisms in liquid chromatography (LC), refined the detailed theory of band broadening in modern LC columns, and contributed to improve column and instrument technologies in both LC and supercritical fluid chromatography.
Dr. Gritti has been invited to give about thirty seminars on diverse topics of chromatographic sciences worldwide. He has delivered over ninety-five invited keynote lectures and published over three hundred peer-reviewed articles. Dr. Gritti was the recipient of the 2013 Chromatographic Society Jubilee Medal, the 2019 JFK Huber Lecture Award, the 2022 Eastern Analytical Symposium Award for Outstanding Achievements in Separation Science, the 2023 Csaba Horvath Memorial Award for propagation of separation sciences throughout the world and co-operation in the development of chromatography in Hungary, and the 2024 A.J.P. Martin Medal.
05:40 pm
In-line Tools for SMB Method Development in Lab Scale Used for Purification of Natural Compounds (OC19)
Dr Yannick KRAUKE KNAUER, Berlin, Germany
Session 4B: Stationary phases 2
Session Chairs
Dr Weston UMSTEAD DAICEL CHIRAL TECHNOLOGIES, West Chester, United States
05:00 pm
High Resolution 3D Imaging to Visualize and Characterize 3D Printed Chromatography Columns (OC20)
Dr Thomas JOHNSON UNIVERSITY COLLEGE LONDON, London, United Kingdom
05:20 pm
Title TBC (OC21)
Dr Nick VECCHIARELLO UNIVERSITY OF VIRGINIA, Charlottesville, United States
05:40 pm
Evaluation of Large-scale Monolith Chromatographic Columns for AAV Capsids Separation (OC22)
Dr Urh CERNIGOJ SARTORIUS BIA SEPARATIONS, Ajdovscina, Slovenia
Dr Urh CERNIGOJ
Dr. Urh Černigoj is Head of Research and Development Laboratory at Sartorius BIA Separations, Slovenia, EU. After obtaining Master�s degree in organic chemistry at Faculty of Chemistry and Chemical Engineering, University of Ljubljana, Slovenia,he started his PhD in Environmental Chemistry at University of Nova Gorica, Slovenia. He obtained his PhD degree in 2007 (Photodegradation of organic pollutants in aqueous solutions catalyzed by immobilized titanium dioxide). For the doctoral thesis he was awarded by Jozef Stefan Golden Emblem Prize for outstanding doctoral thesis in the field of natural sciences. He is employed by Sartorius BIA Seperations from 2010, where he started as a Researcher in the field of monolith surface modification. He was appointed Head of Research and Development in 2020. During last 15 years he has been leading the development of several novel and optimized chromatographic columns and methods on a monolithic platform for purification of large biomolecules, especially nucleic acids and immunoglobulins. He has co-authored cca 48 original scientific papers, 4 book chapters and 6 international patent applications.
06:00 pm
Reception
MILLENIUM HALL
Thursday May 30, 2024
Breakfast Vendor Seminars
07:00 am
Start of parallel sessions
Vendor Seminar by Cytiva
Vendor Seminar by Shimadzu
Vendor Seminar by Thermo Fisher Scientific
Vendor Seminar by Cytiva
07:00 am
Purification of Bispecific Antibodies and Fragments Using the VH3 Interaction
Vendor Seminar by Shimadzu
07:00 am
Journey to Develop Preparative SFC, a Story of Bridging Collaboration to Innovation
Vendor Seminar by Thermo Fisher Scientific
07:00 am
Case Studies with Hydrophobic Interaction and Mixed-Mode Chromatography: Flow-Through Technologies for mAb Polishing.
08:15 am
Opening Remarks
Dr Olivier DAPREMONT SK PHARMTECO, Rancho Cordova, United States
COMMONWEALTH BCD
Session 5: Sustainability
COMMONWEALTH BCD
Session Chair
Dr Olivier DAPREMONT SK PHARMTECO, Rancho Cordova, United States
08:20 am
Enabling Sustainability in Chromatography via Innovation (PL02)
Dr David ROUSH ROUSH BIOPHARMA PANACEA , Colts Neck, United States
Dr David ROUSH
Dr. David Roush is currently CEO/Distinguished Scientist at Roush BioPharma Panacea, LLC (RBP). RBP is an enterprise dedicated to the successful development and optimization of biological products via strategic guidance derived from and leveraging a combination of strong scientific and engineering fundamentals and a plethora of experience. The organization provides scientific and technical guidance/consulting on all aspects of process development (ex. Developability, Control Strategy, Adventitious Agent Control, Modeling), technology development and commercialization enabled via a collaborative business model.
He is actively engaged in the biotechnology community currently serving as a member of the PREP Scientific Advisory Cmte, Organizing Cmte for Recovery of Biological Products Modeling Workshops, Adjunct Professor at University of Delaware Department of Chemical and Biomolecular Engineering, and an ACS Councilor on the Meetings and Expositions Cmte, representing the BIOT Division. Dr. Roush previously served as session chair at PREP and ACS National Meetings (BIOT Division) and on the Northwestern University Master of Biotechnology Program Industrial Advisory Board for a decade, providing feedback on curriculum and mentoring graduate students.
Dr. Roush has more than 30 years of experience in bioprocess development (proteins, natural products and vaccines) directly contributing to the development of five life-saving therapeutics including the natural product/semi-synthetic anti-fungal drug CandidasTM and the immuno-oncology antibody KeytrudaTM while a Distinguished Scientist at Merck BioProcess R&D.; Specific examples include the development of both non-chromatographic and chromatographic purification processes for isolation and purification of an intermediate of CandidasTM which included an in situ solvent recovery step. Dr. Roush�s contributions to the development and commercialization of KeytrudaTM were honored with the ACS BIOT Industrial Biotechnology Award (2016) and ACS Heroes of Chemistry Award (2021). He was selected as an ACS Fellow in 2019 for his combined contributions to the advancement of the Chemical Sciences and associated impact to society as well as ACS.
His experience spans process development for natural products, peptides, proteins, antibody drug conjugates from microbial, mammalian and fungal host spanning end-to end development from Discovery through Clinical Development and Commercialization including scale-up/scale-down and technology transfer. Dr. Roush was one of the pioneers in the development and use of computational biophysics models to support rational development of downstream processes and formulation development, which includes the application of Computational Developability.
Dr. Roush has been actively engaged in advancement of scientific knowledge and the development of new technologies via collaborations with leading academic researchers and consortia (ex. NIIMBL, WuXi, Penn State, RPI, NC State) authoring more than 120 publications and presentations on the topics of primary recovery, adventitious agent control strategy and associated mechanisms, modeling and new technologies for hcp/lipase control. He received his Bachelor of Science degree in Chemical Engineering from Rice University and his PhD in Chemical Engineering from the University of Houston, under the guidance of Dr. Richard Willson. Dr. Roush�s doctoral research �Thermodynamic and Electrostatic Phenomena in the Ion Exchange of Proteins� was the synthesis of experimental and computational electrostatics and thermodynamics to explore protein-surface interactions, including the effects of charge variants and waters of hydration on chromatographic retention.
09:00 am
Solutions for Reduced Carbon Footprint and Direct Freshwater Use in MAB Manufacturing (OC23)
Mrs Anna GRONBERG CYTIVA, UPPSALA, Sweden
Mrs Anna GRONBERG
Anna Gr�nberg, Staff Research Engineer, joined Cytiva (formerly GE Healthcare Life Sciences) in 2003, and since then focused on the development of chromatography resins for capture and polishing of monoclonal antibody (mAb), and related applications. Throughout her career at Cytiva, Anna worked with several producers of mAbs and gained a thorough understanding on how modern technology and solutions enable more efficient biopharmaceutical production. As an expert in industrial mAb purification, Anna is currently dedicated to development of the new Fibro technology and related industrial applications as well as environmental sustainability aspects from an end-user perspective.
09:20 am
ION Exchange as a Sustainable Alternative to Reversed Phase Chromatography for the Purification of Tides Molecules (OC24)
Dr Cecilia UNOSON BIO-WORKS, Uppsala, Sweden
Dr Cecilia UNOSON
Cecilia Unoson works as a Principal Scientist at Bio-Works. She has been with Bio-Works for 6 years and her primary responsibility is application work focusing on customer purifications processes, especially involving so-called �TIDES� molecules (oligonucleotides and peptides). She has a PhD in molecular biology and a background in chemical Engineering.
09:40 am
Introducing Green Flash Chromatography (OC25)
Dr Kenneth JAMES G-FLASH LLC, Newark, United States
Dr Kenneth JAMES
Dr. James received his Ph.D. from the University of Delaware Department of Chemistry and Biochemistry in 1998 with a concentration in Organic Chemistry and Analytical Spectroscopy. During his time at the University, he was involved in the design and manufacture of high-pressure spectroscopy cells for investigations into the decomposition mechanisms of energetic materials using �T-Jump/FT-IR�, Pyrolysis/FT-IR, Mass Spectroscopy and Raman Spectroscopy.
In 1994 he founded Supercritical Fluid Technologies Inc. and holds the position of President and Director of Technology. He is responsible for all research activities, including new product development and developing new applications for supercritical fluid techniques. The company has sought to fill niche markets by developing innovative solutions for demanding extraction, separation, and material processing needs using scCO2.
In 2022 he established G-Flash, LLC as a technology and business development entity, focused on commercializing scCO2 Flash Chromatography (SFCTM) technology for the pharmaceutical Industry. GFCTM offers an alternative to the traditional solvent based Flash Chromatography that uses vast amounts of organic solvents for separations of pharmaceutical compounds.
10:00 am
Coffee Break and Exhibition Sponsored by NanoMicro Technology
MILLENIUM HALL
Session 6: Modelling / Simulations 1
COMMONWEALTH BCD
Session Chair
Dr Siddharth PARIMAL GSK, Philadelphia, United States Prof. Johannes BUYEL BOKU, Vienna, Austria
10:30 am
Going Deep: Understanding Electrostatic Interaction on Strong Cation-Exchanger Via CO-ION Valency Effects (OC26)
Mr Gregor ESSERT TECHNISCHE UNIVERSIT�T M�NCHEN, Garching, Germany
Mr Gregor ESSERT
Gregor Essert holds a bachelor�s and master�s degree in bioprocess engineering from the Technical University of Munich. Currently, he is a doctoral candidate at the Chair of Bioseparation Engineering at the same university, where he applies experimental and theoretical methods to study transport phenomena and surface interactions in ion chromatography. Additionally, Gregor is a founder of a startup project leveraging his expertise to develop software to accelerate downstream processing through proprietary mathematical models. This project is supported by the German Federal Ministry for Economic Affairs and Climate Action.
10:50 am
Displacement Effects on the Purification of GLP-1 Receptor Agonists (OC27)
Dr Andr� GARCIA KROMASIL BY NOURYON, Bohus, Sweden
Dr Andr� GARCIA
Andr� Garcia holds a master�s degree in Analytical Chemistry from the University of S�o Paulo, Brazil, and a PhD in Food Chemistry from the University of Copenhagen, Denmark. Author and coauthor of 10 peer-reviewed papers, Dr. Garcia has more than 15 years of experience within research and education in life sciences. As a scientist at the Product & Application Development Group of Kromasil, by Nouryon, Sweden, he has been working on novel silica-based stationary phases, from their development to application. Among different projects, he has actively contributed to the development of preparative methods for the purification of GLP-1 receptor agonists
11:10 am
On the Challenges of Reactive Chromatography: Estimation of Kinetics and Thermodynamics of Lactose Mutarotation (OC28)
Mr Silvio TRESPI ETH Z�RICH, Z�rich, Switzerland
Mr Silvio TRESPI
Currently PhD student at ETH Z�rich, in the Separation Processes Lab led by Prof. Dr. Marco Mazzotti. He holds a Bachelor (2017) and a Master of Science (2020) both in Chemical Engineering, from Politecnico di Milano, Italy. His research activity deals with chromatography and crystallization process development.
11:30 am
Prediction Precision of Hydrophobic Interaction Chromatography Can Be Improved with a Flexible Isotherm (OC29)
Prof. Johannes BUYEL BOKU, Vienna, Austria
Prof. Johannes BUYEL
After graduating as B. Sc. in 2003 at the RWTH Aachen University (Molecular Biotechnology), Johannes Buyel studied in Sweden (Lund University) and the USA (Fraunhofer Center for Molecular Biotechnology, Newark, Delaware) during his masters before joining the RWTH again for a PhD in 2009. His work focused on recombinant protein expression in plants and their purification, which Johannes continued to investigate as part of his post-Doc in 2013. Starting in 2014, he joined the Fraunhofer Institute for Molecular Biology and Applied Ecology IME as a group leader for model-based high-throughput bio-process development. In 2015 Johannes was promoted to head the department of Bioprocess Engineering (BPE), supervising process development and scale-up. In this position, he supervised projects focusing on the expression of challenging proteins (e.g. containing intrinsically disordered regions), the overall bioprocess integration (e.g. using side-stream biomass) and the modeling of manufacturing processes as well as their digitalization. In 2017 he received a second PhD in bioprocess engineering at the Karlsruhe Institute of Technology and successfully completed his habilitation in biotechnology in 2020 at the RWTH Aachen University. As of 2022, Johannes is a full professor for downstream process development at the University of Natural Resources and Life Sciences, Vienna (BOKU), where he continues to model, design and scale up processes in the context of plant molecular farming.
11:50 am
Influence of CHO Clonal Variations on Polysorbate 20 Degradation Kinetics in Monoclonal Antibody Formulations (OC30)
Dr Tanja SEIBOLD BOEHRINGER INGELHEIM, Biberach, Germany
Dr Tanja SEIBOLD
She was born on November 6, 1991, in Illertissen, Germany. Tanja attended the University of Ulm from 2011 to 2014, where she obtained her Bachelor of Science degree in molecular biology. She continued her studies at the University of Ulm from 2014 to 2017. During this period, she pursued a Master of Science degree in Pharmaceutical Biotechnology. As part of her studies, she had the opportunity to complete her master thesis (2016-2017) at BioVersys AG in Basel focusing on fighting multiresistant bacteria.
In 2017, Tanja embarked on a Ph.D. and Post Doc journey at the University Hospital Ulm, working in the AG Seufferlein within the Internal Medicine department. Her doctoral thesis revolved around exploring the role of small extracellular vesicles (exosomes) in the propagation of inflammation after trauma.
Since 2023, she has been working as a Post Doc at Boehringer Ingelheim. In the Bioprocess Development Biologicals division, her role primarily revolves around studying critical HCPs (Host Cell Proteins) in bioprocess development.
12:10 pm
Lunch and Exhibition
MILLENIUM HALL & VENDOR SEMINAR ROOMS
Lunch Vendor Seminars
12:15 pm
Start of parallel sessions
Vendor Seminar by Agilent Technologies
Vendor Seminar by Gilson
Vendor Seminar by Kromasil� by Nouryon
Vendor Seminar by Sartorius
Vendor Seminar by Agilent Technologies
12:15 pm
Practical Approaches to Purification of Oligonucl�otides and Peptides Using High-Performance Liquid Chromatography
Vendor Seminar by Gilson
12:15 pm
Purification of Active Ingredients - Scaling Up to Process Scale
Vendor Seminar by Kromasil� by Nouryon
12:15 pm
Kromasil Circle of Value: Purification of Peptides using Reversed Phase Preparative Chromatography
Vendor Seminar by Sartorius
12:15 pm
Sartobind� Rapid A: Economical and Technical Considerations of Multi-Batch Use
01:30 pm
Poster Session 2
MILLENIUM HALL
03:00 pm
Start of parallel sessions
Session 7A: Discovery/Development and Manufacturing Small Molecules
Session 7B: Cell and Gene Therapy
Session 7A: Discovery/Development and Manufacturing Small Molecules
Session Chairs
Dr Geoffrey COX B. INDEPENDENT CONSULTANT HPLC, SMB & SFC, Wallingford, United States Dr Gerard ROSSE PIC SOLUTION, San Diego, United States
03:00 pm
A Flexible Approach to Automated High-throughput Purification to Enable Drug Discovery (OC31)
Ms Amber GUILLEN NURIX, San Francisco, United States
Ms Amber GUILLEN
Amber Guillen currently serves as the group leader of High-Throughput (HT) Purification at Nurix Therapeutics. With over ten years of pharmaceutical industry experience, she has developed an expertise in preparative reverse-phase and super-critical fluid chromatography ranging from milligram to multi-gram scales. She specializes in the purification of small molecules in pharmaceutical research, with an emphasis on the relationships between instrumentation, data management, and automation. Her passion is to implement novel and innovative tools to increase productivity in purification and analytical workflows. Amber earned her Bachelor's degree in Chemistry from the University of California, Los Angeles (UCLA).
03:20 pm
Lab Scale Chiral Chromatography: Evolution and Application to Lilly Early Drug Discovery Efforts (OC32)
Mr Eric SEEST ELI LILLY, Indianapolis, United States
Mr Eric SEEST
Eric grew up in central Illinois and did his undergraduate work in chemistry at Bradley University in Peoria. He also spent some time in a PhD organic chemistry program at UC-Irvine before opting out to pursue analytical chemistry interests. Eric joined the pharmaceutical industry back in 1987 with 16 of those years spent with The Upjohn Company/Pharmacia/Pfizer in Kalamazoo, MI before joining Eli Lilly in Indianapolis, IN in 2003. During much of that time, he has been directly involved with chiral chromatography including HPLC/SFC method development and purification (as well as �achiral� support).
He has seen a lot of changes in the technology and approaches to lab scale chromatography support for Discovery and Development chemistry efforts and plans to share some of that experience and insight today. He is most proud of his loving and supportive wife and family (and most recently a couple of grandchildren) and has been very fortunate to meet and learn from a lot of wonderful analytical chemistry colleagues along the journey.
03:40 pm
Innovations in Enhanced SFC: Complex Purification Enabled by Recent Advances in Analytical and Preparative Enhanced Fluid Chromatography (OC33)
Mr Jimmy DASILVA MERCK & CO., INC., Rahway, United States
04:00 pm
Reversed-phase Microscale Isolations of Various Modalities (OC34)
Ms Crystal YE GENENTECH, South San Francisco, United States
Ms Crystal YE
Crystal S. Ye is currently a Scientist at Genentech in South San Francisco. She is a part of the Discovery Chemistry department, where she has been deeply engaged in analytical research and solving purification and separation problems by RP-HPLC and SFC. Outside of her purification work, she is also a part of several committees at Genentech that promote green chemistry and sustainability in the laboratory.
Prior to her work at Genentech, Crystal received a M.S. in Pharmaceutical Chemistry from University of California, Davis under the supervision of Professor Jacquelyn Gervay-Hague. Her thesis focused on the characterization and isolation of glycolipids found in tea (Camellia sinensis).
Session 7B: Cell and Gene Therapy
Session Chairs
Dr John BOWEN SK PHARMTECO, King of Prussia, United States
Jukka Kervinen studied cell & molecular biology and biochemistry at Universities of Jyvaskyla and Helsinki, Finland. As a post-doctoral fellow at the National Cancer Institute, Frederick, Maryland, he researched the structural properties of retroviral proteases using X-ray crystallography. Jukka�s industry background is in the development of purification and analytical methods for monoclonal antibodies, enzymes, and vaccine candidates. He currently manages chromatographic purification processes and their U/HPLC analytics for adeno-associated virus vectors, monoclonal antibodies, and nucleic acids at Tosoh Bioscience.
03:20 pm
Development of a Convective Flow-based Chromatography Step for the Enrichment of Full Adeno-associated Viral Vectors (OC36)
Ms Susanne KONRAD ROCHE DIAGNOSTICS GMBH, Penzberg, Germany
Ms Susanne KONRAD
Susanne studied Biotechnology at University of Applied Sciences in Mannheim, Germany, where she graduated with a diploma in October 2007. Her thesis focused on the investigation of linear gradient elution for the separation of insulin variants using modeling. In January 2008 she started at Roche Diagnostics GmbH, Penzberg, Germany. From 2008 to 2020 she optimized purification processes of various monoclonal antibody formats (monospecific, bispecific, T-cell binding and IL2 containing) in lab scale and robotic scale. Within this time she strongly focused on purification of antibodies using mixed mode chromatography resulting in two patent applications. Since 2020 Susanne has focused on the purification of recombinant viral vectors. Besides her responsibility as a DSP Phase 3 project lead, she has worked on technology activities and on scaling up and transferring processes to production scale.
03:40 pm
Employing Arginine and Trehalose for Improved Recovery in a Two Stage Lentiviral Vector Purification Process (OC37)
Mr Andrew KOCOT RENSSELAER POLYTECHNIC INSTITUTE, Troy, United States
Mr Andrew KOCOT
Andrew Kocot is a 4th year PhD candidate in Professor Cramer's lab at Rensselaer Polytechnic Institute, where he specializes in lentiviral vector downstream processing. Specific aims of the research include development of a novel two-stage chromatographic process that achieves high infectious recovery, exceptional purity, while maintaining colloidal stability. Additionally, nanoflow cytometry is employed for high-throughput particle characterization of process intermediates. Andrew has garnered significant industry experience through engineering and process development roles at leading biotechnology firms, Biogen and KBI Biopharma. He completed his BS in Chemical and Biomolecular Engineering from North Carolina State University.
04:00 pm
Efficient Capture of Adenovirus Serotype 5 from Multi-cell Culture Fluids by Affinity Ligands Targeting Hexon Protein (OC38)
Mr Yuxuan WU NORTH CAROLINA STATE UNIVERSITY, Raleigh, United States
Mr Yuxuan WU
Yuxuan WU is currently a PhD candidate at North Carolina State University, Department of Chemical and Biological Engineering and he received his Bachelor of Polymers Science and Engineering at Zhejiang University, China. His research interest is in peptide ligands for affinity chromatography. Right now, he is working on finding and designing the peptide ligands for the Adenovirus Purification.
04:20 pm
Coffee Break and Exhibition
MILLENIUM HALL
05:00 pm
Start of parallel sessions
Session 8A: Industrial Case Study
Session 8B: Peptides / Oligos
Session 8A: Industrial Case Study
Session Chairs
Dr Alan HUNTER ASTRAZENECA, Gaithersburg, United States Dr Tim PABST ASTRAZENECA, Gaithersburg, United States
05:00 pm
A Platform Strategy for the Development of Polishing Chromatography Steps (OC39)
Dr Steve TIMMICK GSK, Plymouth Meeting, United States
Dr Steve TIMMICK
Steve Timmick received his bachelor's degree in chemical engineering from the University of Delaware in 2012 and a PhD in chemical and biological engineering with Professor Steve Cramer at Rensselaer Polytechnic Institute in 2017. Upon completing his PhD, Steve joined the Biopharm Drug Substance Development group at GSK and has worked on the development of manufacturing processes for several early and late-stage antibody assets. Steve now leads a small team of downstream scientists and is involved in project progression, department strategy, and as a subject matter expert in chromatographic separations.
05:20 pm
Process Intensification of rAAV Using Column-free Continuous Countercurrent Tangential Chromatography (CCTC) (OC40)
Mr Oleg SHINKAZH CHROMATAN, Ambler, United States
05:40 pm
Developing Continuous Multi-column Chromatography Platforms for Virus-like Particles (VLP): A Transition from Batch to Continuous Vaccine Production (OC41)
Dr Kabir DHINGRA MERCK, West Point, United States
Dr Kabir DHINGRA
Kabir is currently a Senior Scientist at Merck and Co. where he is developing next generation continuous chromatography approaches for a range of vaccine products. Prior to joining Merck, he completed his PhD from Rensselaer Polytechnic Institute. His graduate research focused on evaluating molecular interactions between proteins and various chromatographic resin surfaces through a combination of experimental and in-silico techniques. He has extensive experience in developing downstream chromatographic operations for a range of biomolecules such as virus-like particles, nucleic acids, mono/bispecific antibodies, and antibody fragments
06:00 pm
Investigation of Retrovirus Like Particle Clearance Across Operational Ranges for Hydrophobic Interaction Chromatography Steps Using MOCKV� RVLPs (OC42)
Ms Marisa LABANCA BIOGEN, INC., Morrisville, United States
Ms Marisa LABANCA
Marisa Labanca has been working at Biogen in Cambridge, MA and RTP, NC in the Process Biochemistry group developing downstream processes for monoclonal antibodies and fusion proteins for clinical and commercial biologics. Prior to working at Biogen, Marisa spent 18 months at Boston University in the department of periodontology and oral biology researching salivary proteins. She graduated from the medical technology school at the Inselspital in Bern in Switzerland with internships in chemical, histology, cytology, and hematology laboratories.
Session 8B: Peptides / Oligos
Session Chairs
Dr Franklin Eduardo MEJIA FRIAS PFIZER, Andover, United States Dr Mattia SPONCHIONI POLITECNICO DI MILANO, Milano, Italy
05:00 pm
Towards Development of Peptide-based Host Cell Protein Removal Technologies for Biomanufacturing (OC43)
Ms Sobhana Alekhya SRIPADA NORTH CAROLINA STATE UNIVERSITY, Raleigh, United States
Ms Sobhana Alekhya SRIPADA
Sobhana is a 4th year Ph.D. candidate in the Chemical Engineering department from NC State University. As a part of the BioPep and NC-VVIRAL groups, her research focuses on developing novel matrices - using short peptides as ligands - for process-related impurity removal from CHO, HEK293 and Pichia pastoris culture systems. This presentation will provide details of ligand discovery and the development of processes to remove host cell proteins across cultures producing a variety of therapeutic modalities including monoclonal or bispecific antibodies and viral vectors.
05:20 pm
Development of a Custom, High Throughput Purification Platform in Support of Peptide Purification (OC44)
Ms Cristina GROSANU J&J;, Spring House, United States
Ms Cristina GROSANU
My background in purification sciences includes a wealth of experience, particularly in reverse phase HPLC.
I have successfully carried out this technique on small molecules as well as peptides, ranging from microgram to gram quantities.
I have applied my expertise in automated High Throughput Purification systems at Pfizer, BMS, Merck, and Janssen over a 10 year time span.
I played a key role in establishing Pfizer's purification group, which focused on integrating an automated purification platform and implementing a compound management system. During my time at BMS, I took charge of medium to large scale intermediate purification processes. At Merck, my main focus was collaborating closely with the High Throughput Experiment (HTE) group to facilitate a high volume of microscale purification workflows with fast turnaround times. Since joining Janssen in 2019 I contributed to the build of the high throughput purification (HTP) facility at Spring House and offered purification assistance to the chemists involved in the discovery process.
Prior to that, I was a synthetic chemist for over 11 years at Wyeth. I am highly accomplished in multi-step synthesis, reaction optimization and scale up of intermediate compounds, as well as developing SAR to address key biological issues.
05:40 pm
Integrated Model-based Design and Scale-up for a Peptide Chromatographic Purification Process (OC45)
Dr Bhoja KANDELA ELI LILLY SERVICES INDIA PRIVATE LIMITED, Bangalore, India
Dr Bhoja KANDELA
I, Bhoja Kandela, am a chemical engineer by training. I have completed my PhD from IITBombay, India from dept of chemical engineering. My thesis was mainly focused on feasibility and assessment of multifunctional reactors for synthesis of carboxylic esters. I have joined Lilly at LCCI (Lilly capability center India) in 2019. Currently, I am working on model-derived solutions applied on various portfolios for successful scale-up and targeting accelerated development.
06:00 pm
Large Scale Purification of Peptides with MCSGP Under GMP (OC46)
Dr Ralf EISENHUTH BACHEM AG, Bubendorf, Switzerland
07:30 pm
Gala Dinner
ROOM HOWE
Friday May 31, 2024
07:55 am
Opening Remarks
Dr Olivier DAPREMONT SK PHARMTECO, Rancho Cordova, United States
ROOM COMMONWEALTH BCD
08:00 am
Start of parallel sessions
Session 9A: Modeling / Simulations 2
Session 9B: Natural Products
Session 9A: Modeling / Simulations 2
Session Chairs
Dr Alessandro BUTTE DATAHOW, Zurich, Switzerland Dr Lucas KIMERER GLAXOSMITHKLINE, Charlottesville, United States
08:00 am
Hybrid Models and Knowledge Transfer for Biopharmaceutical Processes (OC47)
Prof. Massimo MORBIDELLI ARISTOTLE UNIVERSITY OF THESSALONIKI, Thessaloniki, Greece
08:20 am
A Machine Learning Methodology for Resin Screening in Protein a Chromatography (OC48)
Ing Andrea GALEAZZI IMPERIAL COLLEGE LONDON, London, United Kingdom
Ing Andrea GALEAZZI
Andrea Galeazzi is a chemical engineer who has completed his PhD studies at Politecnico di Milano, focusing on data-driven and machine learning techniques to improve processes in the chemical industry, particularly in oil regeneration plants. He is now working at Imperial College London in Dr. Maria Papathanasiou's group, researching strategies for data-driven design space identification for Quality by Digital Design and flexibility purposes in the bio-pharma industry.
08:40 am
All-atom Modelling of Methacrylate-based Multi-modal Chromatography Resins for Isotherm Parameter Prediction (OC49)
Mr Tim BALLWEG KARLSRUHER INSTITUTE OF TECHNOLOGY, Karlsruhe, Germany
Mr Tim BALLWEG
Tim Ballweg, currently a doctoral researcher at the Institute of Functional Interfaces, Karlsruhe Institute of Technology (KIT), has been contributing to the institute since 2021. His research primarily revolves around the virtual design of adsorbent materials, specifically concentrating on methacrylate-based chromatography resins. Before embarking on this research journey, Tim gained practical experience through an internship at Tosoh Bioscience in Darmstadt in 2018. Here, he gained skills in high-throughput chromatography for process development.
Academically, Tim holds a Master of Science in Biochemical Engineering from the Karlsruhe Institute of Technology.
Throughout his academic journey, Tim has consistently engaged in research and development in the field of biochemical engineering, with a particular focus on high throughput experiments and modeling in chromatography.
09:00 am
Spatial Discontinuous Galerkin Spectral Element Method for a Family of Chromatography Models in Cadet (OC50)
Mr Johannes SCHM�LDER FORSCHUNGSZENTRUM J�LICH GMBH, J�lich, Germany
Mr Johannes SCHM�LDER
Johannes Schm�lder holds a Master of Science from the
Friedrich-Alexander University Erlangen N�rnberg.
Since 2020, he has been a member of the Modeling and Simulation Group at
the Biotechnology Institute of the Research Center J�lich.
His primary research areas involve coordinating the development of the
CADET software, modeling and optimizing chromatographic processes, and
instructing on the fundamentals of research software engineering and
data management.
Session 9B: Natural Products
Session Chair
Dr Chiara DE LUCA UNIVERSIT� DEGLI STUDI DI FERRARA, Ferrara, Italy
Dr Chiara DE LUCA
Chiara De Luca has defended her PhD thesis in February 2021 at the University of Ferrara (Italy). She is currently employed as a Post-Doc in the same University. During her PhD she spent a period at ETH Zurich (Switzerland) working on continuous purification techniques in the group of Prof. Massimo Morbidelli. Her main research interests lie in the field of biomolecules analysis and purification through LC, with a special attention towards green approaches (alternative solvents, single column and continuous multicolumn prep-LC, etc).
08:00 am
Advancing Rapeseed Protein Purification: A Continuous Chromatographic Approach for Napin and Cruciferin (OC51)
Mr Jonas ARNECKE UNIVERSITY OF APPLIED SCIENCES MANNHEIM, Mannheim, Germany
Mr Jonas ARNECKE
Jonas Arnecke completed his B.Sc. in Biotechnology and his M.Sc. in Bioprocess Development at the Mannheim University of Applied Sciences (Germany). Currently he is a PhD student at the Institute of Biochemistry at the University of Applied Sciences Mannheim (Germany) and he is doing research on the purification of plant proteins and investigating their biopharmaceutical properties.
08:20 am
Counter-current Chromatography for Efficient Lignin Monomer-monomer and Monomer-oligomer Separations from Lignin Oil Derived from Reductive Catalytic Fractionation (OC52)
Dr Manar ALHERECH NATIONAL RENEWABLE ENERGY LABORATORY, Golden, United States
Dr Manar ALHERECH
Manar Alherech is a postdoctoral researcher in the Separations Group at NREL. His interests focus on the application of liquid-liquid chromatographic techniques, specifically centrifugal partition chromatography (CPC) and countercurrent chromatography (CCC), to the isolation of valuable compounds.
08:40 am
Optimization For the Separation of Oligosaccharides by SMB (OC53)
Ms Xiaoqing BAO JOPE TECHNOLOGY CO., LTD , Kaohsiung, Taiwan
Ms Xiaoqing BAO
Xiaoqing BAO is an R&D; manager at JOPE Technology Company. JOPE specializes
in developing processes, engineering design, and manufacturing equipment for
preparative and SMB chromatography eluted either by liquid or by supercritical fluid
(SF). Xiaoqing received a master's degree in Pharmacy from WENZHOU Medical
University in China. She was a research assistant at I-Shou University in Taiwan for
four years, and learned the application of SMB/SF-SMB on pharmaceutical and
nutraceutical separation. Her current research focuses on the separation of active
ingredients from natural products, chiral separation, and biorefinery for biomass using
SMB or SF-SMB
09:00 am
Green Purification of CROCIN-I from Saffron Through the Combination of Multicolumn Countercurrent Chromatography and Ethanol (OC54)
Dr Chiara DE LUCA UNIVERSIT� DEGLI STUDI DI FERRARA, Ferrara, Italy
Dr Chiara DE LUCA
Chiara De Luca has defended her PhD thesis in February 2021 at the University of Ferrara (Italy). She is currently employed as a Post-Doc in the same University. During her PhD she spent a period at ETH Zurich (Switzerland) working on continuous purification techniques in the group of Prof. Massimo Morbidelli. Her main research interests lie in the field of biomolecules analysis and purification through LC, with a special attention towards green approaches (alternative solvents, single column and continuous multicolumn prep-LC, etc).
09:40 am
Start of parallel sessions
Session 10A: Downstream Bio Process
Session 10B: Process Development and Optimization
Session 10A: Downstream Bio Process
Session Chair
Dr Nick VECCHIARELLO UNIVERSITY OF VIRGINIA, Charlottesville, United States
09:40 am
Optimizing Bioprocess Efficiency: A Toolbox Approach to Downstream Intensification and Facility Integration (OC55)
Dr Matthias HECHT BOEHRINGER INGELHEIM, Biberach, Germany
10:00 am
Case Studies: Downstream Process Development and Platform Evolution for Fragmented-antibody-based Protein Therapeutics for Inhalation Delivery (OC56)
Dr Haibin LUO ASTRAZENECA, Gaithersburg, United States
10:20 am
Scale-Bridging Separation of Gold Nanoclusters and Plasmonic Nanoparticles by Liquid Chromatography (OC57)
Antibody Capture from Non-clarified Cell Broth Based on Superparamagnetic Protein a Functionalized Beads (OC58)
Dr Nils BRECHMANN MAGIC BIOPROCESSING, Uppsala, Sweden
Session 10B: Process Development and Optimization
Session Chair
Prof. Shuichi YAMAMOTO YAMAGUCHI UNIVERSITY, Ube, Japan
Prof. Shuichi YAMAMOTO
After completing his Ph. D study at Kyoto University, he joined Yamaguchi University. He is
currently Professor for research. He is also working as the downstream process group leader for a
National Consortium, Manufacturing Technology Association of Biologic (MAB).
His research interests are in the field of bio-chemical engineering, food engineering and mass
transfer in addition to process chromatography. His chromatography model better known as
"Yamamoto Model/Method" has been employed as a standard method and implemented in
commercial/open source software.
Currently he is Past President of Asian Pacific Confederation of Chemical Engineering (APCChE),
and a Council Member of World Chemical Engineering Council (WCEC) as a representative of the
Society of Chemical Engineers, Japan (SCEJ). He is also Division Chair of Bioprocess and
Bioseparation Engineering Division of AFOB (Asian Federation of Biotechnology).
09:40 am
A Method for Determining the Optimum Temperature for a Polyphenol Separation Process by Polymer-resin Based Reversed Phase Chromatography (OC59)
Prof. Shuichi YAMAMOTO YAMAGUCHI UNIVERSITY, Ube, Japan
Prof. Shuichi YAMAMOTO
After completing his Ph. D study at Kyoto University, he joined Yamaguchi University. He is
currently Professor for research. He is also working as the downstream process group leader for a
National Consortium, Manufacturing Technology Association of Biologic (MAB).
His research interests are in the field of bio-chemical engineering, food engineering and mass
transfer in addition to process chromatography. His chromatography model better known as
"Yamamoto Model/Method" has been employed as a standard method and implemented in
commercial/open source software.
Currently he is Past President of Asian Pacific Confederation of Chemical Engineering (APCChE),
and a Council Member of World Chemical Engineering Council (WCEC) as a representative of the
Society of Chemical Engineers, Japan (SCEJ). He is also Division Chair of Bioprocess and
Bioseparation Engineering Division of AFOB (Asian Federation of Biotechnology).
10:00 am
Predictability of Two-Component Protein Column Adsorption with Isotherm Models using Batch Experiments (OC60)
Prof. Rainer HAHN BOKU VIENNA, Vienna, Austria
Prof. Rainer HAHN
Rainer Hahn, PhD, is an Associate Professor at the Institute of Bioprocess Science and Engineering (IBSE) at BOKU University, Vienna, Austria. His research focuses on separation of biomolecules from fermentation broths and covers a broad range of unit operations like centrifugation, homogenization, filtration, ultrafiltration and chromatographic techniques. Research activities involve preparative separations as well as mechanistic modelling and engineering aspects. He conducted several benchmark studies comparing commercial chromatography media, e.g. Protein A affinity, IEX and HIC sorbents, and elucidated multi-component adsorption phenomena and mass transfer mechanisms on different chromatography media. Prof. Hahn has published more than 90 peer-reviewed papers and book chapters in the area of bioprocessing engineering and chromatography.
10:20 am
Improved Process Development and Robust Scale-Down Models for Live Virus and Virus Like Particle Purification using Automated High Throughput Chromatography Platform (OC61)
Ms Sheng-Ching WANG MERCK, West Point, United States
10:40 am
Automated System for Modelling, Calibration and Optimization of Chromatographic Separations of Biologics (OC62)
Prof. Bernt NILSSON LUND UNIVERSITY, Lund, Sweden
11:00 am
Coffee Break
COMMONWEALTH FOYER
11:20 am
Poster Awards
Session 11: Alternative Techniques, Membranes, Monolith
ROOM COMMONWEALTH BCD
Session Chairs
Prof. Ruben CARBONELL NC STATE UNIVERSITY, Raleigh, United States Dr Ales PODGORNIK COBIK, Slovenia
11:30 am
Ionic Strength Effects in Protein a Affinity Membrane Chromatography (OC63)
Mr Oussama MENOUAR MENARI GHEZIEL MANNHEIM UNIVERSITY OF APPLIED SCIENCES, Mannheim, Germany
Mr Oussama MENOUAR MENARI GHEZIEL
Oussama Menouar Menari Gheziel hold a B.Sc. in Biotechnology from the University of Granada (Spain) and an M.Sc. in Biotechnology from the Mannheim University of Applied Sciences (Germany) where he is currently doing research on the mAbs downstream processing as a phD candidate.
11:50 am
High Throughput Evaluation of Membrane Chromatographic Selectivities (OC64)
Mr Eric DENBAUM RENSSELAER POLYTECHNIC INSTITUTE, Troy, United States
Mr Eric DENBAUM
My name is Eric Denbaum and I am a 5th year PhD student in Steven Cramer's bio-separations lab at RPI. My work focuses on high throughput screening of chromatographic ligands for the purposes of process development and novel ligand discovery.
12:10 pm
Optimizing Plasmid DNA Purification: Anion Exchange Membrane Chromatography with Salt Modulation (OC65)
Ms Pauline BEWOSCH HS MANNHEIM , Mannheim, Germany
Ms Pauline BEWOSCH
Pauline Bewosh hold a Bachelor's degree in Biotechnology from Mannheim University of Applied Sciences, Germany. Currently, she is pursuing my Master's degree, specializing in Bioprocess Development, also at the University of Applied Sciences. Her master's thesis focuses on examining the application of membrane chromatography for the capture and polishing of pDNA and the overall effect of salt on pDNA purification.
12:30 pm
Characterization of Novel High-performance Chromatography Membranes and Devices for Product Capture (OC66)
Dr Joseph LAVOIE NC STATE UNIVERSITY, Cary, United States
Dr Joseph LAVOIE
Joe Lavoie is a Senior Scientist at the Biopharmaceutical Manufacturing Training and Education Center (BTEC) at NC State University. Prior to this he was Post-Doctoral Researcher at NC State, a Product Development Engineer at Glen Raven, a fabric manufacturing company, and he worked at the US Army Natick Soldier Research, Development and Engineering Center. He has a Ph.D. and an M.S. in Chemical Engineering from NC State University and a B.S. in Chemical Engineering from Northeastern University."
12:50 pm
Size-exclusion Mixed-mode (SEMM) Media for Polishing Antibodies and Viral Vectors in Flow-Through Mode (OC67)
Dr Stefano MENEGATTI NC STATE UNIVERSITY & LIGATRAP TECHNOLOGIES, Raleigh, United States